Orange Juice, A Natural Source for Enhancing in Vitro Regeneration in Saccharum Spp.

Orange juice is a natural source of various vitamins especially Vitamin C, as well as sugar, potassium, thiamine, folate, flvonoids and an antioxidant hesperidins. Therefore the effect of orange juice on in vitro regeneration of sugarcane was investigated. For this study, genotype S-2003-us-359 was selected, in which we had already established in vitro regeneration system and a media combination had been selected with highest regeneration potential. In this study, this media combination was used as control and also modified the regeneration medium of this media combination by adding orange juice and dry milk. An increase in regeneration potential of this media combination was observed by adding orange juice in regeneration medium.