Caught in the act. The enzyme 4-amino-4-deoxychorismate synthase (ADCS) catalyses the amination of chorismate by using glutamine as the ammonia source. ADCS consists of a chorismate-aminating subunit, PabB, and a glutamine amidotransferase subunit, PabA. Using electrospray mass spectrometry, we have directly detected the formation of enzyme-bound covalent intermediates on both PabB (as shown in figure) and PabA.
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Bulloch et al. (2005) studied this question.
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