The influence of forces on the particles other than the applied cross flow is studied in hollow fiber flow field‐flow fractionation (HF5). Such forces can be of either attractive or repelling in nature. It is shown by injecting polystyrene latex beads (0.091 μm in diameter) at different cross flows and by using eluents with different ionic strengths, that there will be disturbances in the concentration profile of the sample irrespective of the type of interacting forces. The retention time and peak broadening were either increased or decreased compared to the theoretical value, depending on the nature of the dominating interaction force. It is, nevertheless, possible to minimize the total interaction energy between particles and between the particles and the channel wall by choosing a suitable ionic strength.
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Carlshaf et al. (1991) studied this question.
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