Key result
Upregulation of RGC-32 was observed in patients with dilated cardiomyopathy compared to healthy controls, contributing to an increase in Th17 and a decrease in Treg cells.
Why the study?
Does RGC-32 upregulation contribute to the imbalance of Treg/Th17 cells in patients with dilated cardiomyopathy?
Case-Control
Does RGC-32 upregulation contribute to the imbalance of Treg/Th17 cells in patients with dilated cardiomyopathy?
Upregulation of RGC-32 contributes to the pathogenic imbalance of Treg/Th17 cells in patients with dilated cardiomyopathy.
RGC-32 upregulation was associated with Treg/Th17 imbalance in DCM; hypothesis-generating for targeted interventions pending prospective validation.
BACKGROUND/AIMS: The imbalance of Treg/Th17 cells plays important role in the pathogenesis of dilated cardiomyopathy (DCM). Response gene to complement (RGC)-32 is a cell cycle regulator that plays an important role in cell proliferation. We evaluated whether the upregulation of RGC-32 was implicated in the homeostasis of Treg/Th17 cells in DCM. METHODS: The levels of plasma RGC-32, IL-17 and TGF-β1, and the frequencies of circulating CD4+ RGC-32+ T cells, Th17 and Treg cells in patients with DCM were determined by Cytokine-specific sandwich ELISA and the flow cytometer (FCM), respectively. RESULTS: A significant elevation of plasma RGC-32 in patients with DCM compared with healthy control (HC) subjects was observed. This upregulation was associated with an increase in frequency of Th17 and a decrease in frequency of Treg cells. To further assessed the role of RGC-32, we investigated the effects of RGC-32 up- or down-regulation on frequencies of Th17 and Treg cells in peripheral blood mononuclear cells (PBMCs) from subjects. Importantly, overexpression of RGC-32 was accompanied by an augmentation of Th17 and a reduction of Treg expression. CONCLUSION: In summary, our study demonstrated the up-regulation of RGC-32 contributed to the imbalance of Treg/Th17 cells in patients with DCM.
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Li et al. (2017) conducted a case-control in Dilated cardiomyopathy (DCM). RGC-32 upregulation vs. Healthy controls was evaluated on Frequencies of Th17 and Treg cells. Upregulation of RGC-32 was observed in patients with dilated cardiomyopathy compared to healthy controls, contributing to an increase in Th17 and a decrease in Treg cells.
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