Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

Lipopolysaccharide (LPS) of Pseudomonas morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage inactivation (PI50 = 0.05 mug LPS ml-1), whereas LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (pH 7.4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to I mg ml-1, but produced weak chlorosis in bean and tobacco at 2 mg ml-1: no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined.