15N‐NMR spectroscopy was used to follow nitrogen assimilation and amino acid biosynthesis in the intact mycelium and extracts of the symbiotic fungus Cenococcum graniforme. The primary incorporation of 15NH4 + appears to proceed via the glutamine synthetase activity. Glutamine, γ‐aminobutyrate, alanine and arginine were the main labelled nitrogenous pools. The quenching of arginine in intact mycelium suggest that the amino acid is associated with a cellular component which restricts its rotational freedom.
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Francis Martin (1985) studied this question.