Key result
Correlated breast cancer ILT4 and IL-10 expression is linked to fewer lymphocytes and increased metastasis.
Cross-Sectional (n=117)
No
ILT4 and IL-10 are highly expressed in primary breast cancer and correlate with reduced tumor-infiltrating lymphocytes and increased lymph node metastasis, suggesting a role in immune evasion.
ILT4/IL-10 expression may mark immune evasion in breast cancer; hypothesis-generating and requires prospective validation before clinical consideration.
BACKGROUND: Immunoglobulin-like transcript 4 (ILT4) is an inhibitory molecule involved in immune response and has recently been identified to be strongly inducible by IL-10. The aim of the present study was to examine the associations of ILT4 expression with clinicopathological characteristics and IL-10 expression in primary ductal and lobular breast cancer. METHODS: We studied the expression of ILT4 in 4 cancer cell lines, 117 primary tumor tissues and 97 metastatic lymph nodes from patients with primary ductal and lobular breast cancer by reverse transcription-polymerase chain reaction, western blot or immunohistochemistry analysis. Additionally, IL-10 expression was also investigated using immunohistochemistry in primary tumor tissues. Then the relationship between ILT4 expression and clinicopathological characteristics/IL-10 expression was evaluated. RESULTS: ILT4 was highly expressed in all 4 human breast cancer cell lines on both mRNA and protein levels. In primary tumor tissues, ILT4 or IL-10 was expressed in the cell membrane, cytoplasm, or both; the positive rate of ILT4 and IL-10 expression was 60.7% (71/117) and 80.34% (94/117), respectively. ILT4 level was significantly correlated with IL-10 (r =0.577; p<0.01). Furthermore, the expression of ILT4 or IL-10 was associated with less number of Tumor Infiltrating Lymphocytes (TILs) (p=0.004 and 0.018, respectively) and more lymph node metastasis (p=0.046 and 0.035, respectively). CONCLUSION: Our data demonstrated the association of ILT4 and IL-10 expression in human breast cancer, suggesting their important roles in immune dysfunction and lymph node metastases. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1692652692107916.
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Liu et al. (2014) conducted a cross-sectional in Primary ductal and lobular breast cancer (n=117). ILT4 and IL-10 expression vs. Negative or low expression was evaluated on Correlation between ILT4 and IL-10 expression in primary tumor tissues (r = 0.577, p=<0.01). In primary breast cancer tissues, ILT4 expression was significantly correlated with IL-10 expression (r = 0.577, p < 0.01), and both were associated with fewer tumor-infiltrating lymphocytes and increased lymph node metastasis.
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