Endogenous y-aminobutyric acld (GABA) from aqueous (1 M KCI/HCI) or organic (methanol) homogenates of rat brain tissue has been determined by reversed-phase isocratic high-performance liquid chromatography (HPLC) (0.1 M H,PO,/acetonitrile, 4:6 at pH 2.8) with fluorometric detection of the corresponding o-phthalaidehyde derivative at 360 nm (excitation) and 440 nm (emission). Calibration standards were prepared with deuteriated GABA and/or aminovaleric acid (AVA). The precision of the method was 3.2% and the detection limit was established at 64 pg of GABA on column allowing the determination of GABA at the level of 1.7 ng/mg of tissue. The content of GABA In brain was 227.2 f 11.1 ( n = 10) pg/g and in cerebellum 110.3 f 13.9 ( n = 10) pg/g. Average recovery was 91.4 f 11.6%. The method was validated by comparison of these results with those obtained by analyzing brain homogenates of 10 rats by capillary gas chromatography-mass spectrometry, direct thermospray high-performance liquid chromatography-mass spectrometry, and gas chromatography with electron capture detection.
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Suñol et al. (1988) studied this question.
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