An exchange is better than a rest: An NMR screening method is described which facilitates the screening of compound mixtures for components that bind protein drug targets. The approach allows detection of active molecules thanks to a “probe” molecule, which competes with a library of potential ligands for binding sites on a targeted protein. The whole spectrum of active ligands, including those displaying either very fast or very slow exchange behavior, are equally susceptible to detection by this method.
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Siriwardena et al. (2002) studied this question.