Ises A. Abrahamsohn, Alejandro M. Katzin, Regina V. Milder, A Method for Isolating Trypanosoma cruzi Amastigotes from Spleen and Liver Using Two-Step Discontinuous Gradient Centrifugation, The Journal of Parasitology, Vol. 69, No. 2 (Apr., 1983), pp. 437-439
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Abrahamsohn et al. (1983) studied this question.