Comparative transcriptome analysis between heat-tolerant and sensitive Pyropia haitanensis strains in response to high temperature stress

Pyropia haitanensis farms in South China often suffer from sustained high temperatures in autumn, which is the early seeding period, resulting in disease, premature senility, and eventual decay, ultimately leading to a substantial reduction in yield. However, the molecular mechanisms underlying the response to high temperature stress in P. haitanensis remain unknown. In the present study, we used the Illumina sequencing platform to examine the transcriptional profiles of heat-tolerant (THT) and heat-sensitive (WHT) P. haitanensis strains exposed to different durations of high temperature stress. THT exhibited more differentially expressed genes and greater fold-changes in transcripts under sustained temperature stress, suggesting that this strain is better able to increase transcriptional regulation in response to high temperature stress than WHT. Furthermore, heat stress induced different expression patterns in the thalli of the two strains. THT was able to maintain or increase the activities of energy metabolism, antioxidant systems, and phosphatidylinositol signal transduction to resist heat stress, while these processes were dramatically reduced in WHT, resulting in the thalli being unable to survive under sustained high temperatures. The present data provide a better understanding of the mechanisms underlying the response of P. haitanensis to high temperature stress, which may facilitate the development of technologies and breeding strategies for improving thermotolerance in Pyropia . • Z-61 (THT) of Pyropia haitanensis has higher thermotolerance than wild-type (WHT). • THT exhibits more differentially expressed genes and greater fold-changes than WHT. • Heat stress induces different expression patterns in two P. haitanensis strains. • THT exhibits a more positive transcriptional response to heat stress.