Detection of Fusarium oxysporum f. sp. v asinfectum race 3 by single-base extension method and allele-specific polymerase chain reaction

Allele specific (AS) SNP primers were developed for rapid detection of Fusarium oxysporum f.sp. vasinfectum (FOV) race 3. These primers were designed based on single nucleotide polymorphisms of partial sequence alignment of the β-tubulin (BT) gene from several FOV races. The primers showed high specificity for FOV race 3, and exclusively amplified PCR products from FOV race 3 DNAs and total genomic DNAs of FOV race 3-infected plant tissues. These primers did not produce any amplification products for races 1, 2, 4, 6, 7 and 8 of FOV or other FOV and Fusarium species from infected plant tissues. The assay is useful for specific and rapid detection of Fusarium oxysporum f. sp. vasinfectum race 3.