Key result
High-level combined EMF exposure alters cerebral HSP 90 and 70 levels without affecting caspase-3.
Combined electromagnetic signals at 900 and 2450 MHz induce cellular stress in rat nervous tissue through mechanisms that are not additive compared to single signals.
Hypothesis-generating for non-additive RF stress in rodent cortex; leaves open human relevance and should not change exposure guidelines.
// Alberto López-Furelos 1 , José Manuel Leiro-Vidal 2 , Aarón Ángel Salas-Sánchez 3 , Francisco José Ares-Pena 3 and María Elena López-Martín 1 1 Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain 2 Institute of Alimentary Analysis, University of Santiago de Compostela, Santiago de Compostela, Spain 3 Department of Applied Physics, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela, Spain Correspondence to: María Elena López-Martín, email: // Keywords : heat shock proteins, specific absorption rate, caspase-3, multiple electromagnetic fields, cerebral cortex, Pathology Section Received : October 30, 2015 Accepted : July 16, 2016 Published : August 31, 2016 Abstract Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous systems. In this study, we investigated the non-thermal SAR (Specific Absorption Rate) in the cerebral or cerebellar hemispheres of rats exposed in vivo to combined electromagnetic field (EMF) signals at 900 and 2450 MHz. Forty rats divided into four groups of 10 were individually exposed or not exposed to radiation in a GTEM chamber for one or two hours. After radiation, we used the Chemiluminescent Enzyme-Linked Immunosorbent Assay (ChELISA) technique to measure cellular stress levels, indicated by the presence of heat shock proteins (HSP) 90 and 70, as well as caspase-3-dependent pre-apoptotic activity in left and right cerebral and cerebellar hemispheres of Sprague Dawley rats. Twenty-four hours after exposure to combined or single radiation, significant differences were evident in HSP 90 and 70 but not in caspase 3 levels between the hemispheres of the cerebral cortex at high SAR levels. In the cerebellar hemispheres, groups exposed to a single radiofrequency (RF) and high SAR showed significant differences in HSP 90, 70 and caspase-3 levels compared to control animals. The absorbed energy and/or biological effects of combined signals were not additive, suggesting that multiple signals act on nervous tissue by a different mechanism.
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López-Furelos et al. (2016) studied this question. Combined electromagnetic field (EMF) signals vs. No radiation exposure was evaluated on Cellular stress levels (HSP 90 and 70) and caspase-3-dependent pre-apoptotic activity. Exposure to combined electromagnetic field signals at 900 and 2450 MHz caused significant differences in HSP 90 and 70 levels, but not caspase-3, in the cerebral cortex at high SAR levels.
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