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January 1, 2001Bioscience Biotechnology and BiochemistryOpen Access

Flagellin as a Biomarker for Bacillus subtilis Strains; Application to the DB9011 Strain and the Study of Interspecific Diversity in Amino-acid Sequences

Yoshiya Asano H. Onishi Kentaro TAJIMA Takao Shinozawa

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Abstract

Polymerase chain reaction (PCR) for the flagellin central domain coding region (FCD-PCR) was applied to the detection and discrimination of Bacillus subtilis DB9011, a strain with useful functions in agriculture. Cross-reactions were observed in 4 B. subtilis strains with similar flagellin genes (hag). Alignment of partial amino-acid sequences of flagellin and the results of PCR for the 16S/23S rRNA spacer in 11 B. subtilis strains suggested the presence of a group including strains with antifungal activity (DB9011 and others).

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Asano et al. (2001) studied this question.

synapsesocial.com/papers/6aa23a4e57affa4c5d46f659 https://doi.org/10.1271/bbb.65.1218

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