Linkage analysis of half-sib groups, a note on the use of double intercrosses

The principles of linkage analysis of hell-sib groups are briefly outlined. It is pointed out that in the presence of linkage disequilibrium, the use of double intercrosses leads to biased estimates of recombination frequencies. Nevertheless, it is concluded that, with some exceptions, these matings can be used for the detection of linkage, but they should not be used for the estimation of linkage.