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September 6, 2021DevelopmentOpen Access

Prrx1b restricts fibrosis and promotes Nrg1-dependent cardiomyocyte proliferation during zebrafish heart regeneration

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Key result

Prrx1b is required for scar-free regeneration of the zebrafish heart, as its loss results in excessive fibrosis and impaired cardiomyocyte proliferation.

Why the study?

The mechanisms that drive permanent scarring versus scar-free regeneration following cardiac injury are not well understood.

Does Prrx1b regulate fibrosis and cardiomyocyte proliferation during heart regeneration?

Population

Zebrafish and human fetal epicardial-derived cells

Comparison

Loss of Prrx1b or Prrx1 manipulation vs controls

Design

In vivo and in vitro experimental study

Authors

DBDennis E. M. de BakkerMBMara BouwmanEDEsther Dronkers

Discussion

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Member takes

Overview

Prrx1b modulation merits testing in mammalian models; leaves open translation to human cardiac repair.

Structured PICO

Does Prrx1b regulate fibrosis and cardiomyocyte proliferation during heart regeneration?

P
Population
Zebrafish heart injury model and human fetal epicardial-derived cells
I
Intervention
Loss of Prrx1b (in vivo) or Prrx1 manipulation (in vitro)
C
Comparator
Wild-type or control
O
Outcome
Fibrosis and cardiomyocyte proliferation during heart regenerationsurrogate

Prrx1b is a key transcription factor that balances fibrosis and regeneration in the injured zebrafish heart by restricting TGFβ and promoting Nrg1-dependent cardiomyocyte proliferation.

Cite This Study

Bakker et al. (2021) studied Cardiac injury. Prrx1b was evaluated on Fibrosis and cardiomyocyte proliferation. Prrx1b is required for scar-free regeneration of the zebrafish heart, as its loss results in excessive fibrosis and impaired cardiomyocyte proliferation.

synapsesocial.com/papers/6aa272fb74fc654bd032327chttps://doi.org/10.1242/dev.198937
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Human fetal and adult epicardial-derived cells: a novel model to study their activation2016 · 62 citations
  2. 2Pericardial Mesoderm Generates a Population of Coronary Smooth Muscle Cells Migrating into the Heart along with Ingrowth of the Epicardial Organ1996 · 629 citations
  3. 3Functional Heterogeneity within the Developing Zebrafish Epicardium2020 · 86 citations