What does this research mean for the field?

A novel retention time and MS2-guided HILIC-Orbitrap workflow enables rapid, highly repeatable metabolite screening in under 9 minutes while significantly reducing false-positive annotations. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Develop a rapid and sensitive metabolomics screening workflow that reduces false-positive annotations and analysis time for polar metabolites in complex biological samples.

September 10, 2026Journal of the American Society for Mass Spectrometry

Bridging Speed and Confidence in Metabolomics: A Retention Time and MS2-Guided HILIC-Orbitrap Workflow

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Authors

JRJonas Rösler CKConstantin P. Krempe FCFeyza Cansiz

Discussion

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Overview

Analytical study demonstrates an accelerated HILIC-Orbitrap workflow for complex biological samples, highlighting reduced false-positive annotations in rapid metabolic profiling.

Key Points

Develop a rapid and sensitive metabolomics screening workflow that reduces false-positive annotations and analysis time for polar metabolites in complex biological samples.
Optimized a superficially porous sulfobetaine hydrophilic interaction liquid chromatography (HILIC) method to resolve polar metabolites in under 9 minutes.
Coupled chromatographic separation to a Q-Exactive Plus Orbitrap utilizing an inclusion-list-driven data-dependent acquisition (DDA) full-scan mode.
Constructed an open-source retention time database cataloging approximately 400 metabolites across central metabolic pathways.
Resolved polar metabolites from complex samples in less than 9 minutes with demonstrated repeatability and sensitivity.
Substantially lowered false-positive metabolite annotations through retention time filtering and targeted MS2 acquisition, enabling a streamlined data analysis pipeline.

Cite This Study

Rösler et al. (2026) studied this question.

synapsesocial.com/papers/6aa27b0c58559d80afc74145 https://doi.org/10.1021/jasms.6c00203

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September 10, 2026Journal of the American Society for Mass Spectrometry

Bridging Speed and Confidence in Metabolomics: A Retention Time and MS2-Guided HILIC-Orbitrap Workflow

View Full Paper

Ask AI

Bookmark

Authors

JRJonas Rösler CKConstantin P. Krempe FCFeyza Cansiz

Discussion

Member takes

Overview

Analytical study demonstrates an accelerated HILIC-Orbitrap workflow for complex biological samples, highlighting reduced false-positive annotations in rapid metabolic profiling.

Key Points

Develop a rapid and sensitive metabolomics screening workflow that reduces false-positive annotations and analysis time for polar metabolites in complex biological samples.
Optimized a superficially porous sulfobetaine hydrophilic interaction liquid chromatography (HILIC) method to resolve polar metabolites in under 9 minutes.
Coupled chromatographic separation to a Q-Exactive Plus Orbitrap utilizing an inclusion-list-driven data-dependent acquisition (DDA) full-scan mode.
Constructed an open-source retention time database cataloging approximately 400 metabolites across central metabolic pathways.
Resolved polar metabolites from complex samples in less than 9 minutes with demonstrated repeatability and sensitivity.
Substantially lowered false-positive metabolite annotations through retention time filtering and targeted MS2 acquisition, enabling a streamlined data analysis pipeline.

Cite This Study

Rösler et al. (2026) studied this question.

synapsesocial.com/papers/6aa27b0c58559d80afc74145 https://doi.org/10.1021/jasms.6c00203

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