What does this research mean for the field?

Column-based RNA extraction provides the highest and most consistent detection probability for grapevine fanleaf virus (GFLV) in its nematode vector Xiphinema index, with detection efficiency increasing alongside nematode sample size. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To compare the efficacy of four sample preparation strategies for detecting grapevine fanleaf virus (GFLV) in its nematode vector, Xiphinema index, using RT-PCR.

September 10, 2026European Journal of Plant PathologyOpen Access

Comparative analyses of molecular detection protocols for grapevine fanleaf virus (GFLV) in Xiphinema index

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Authors

JPJulija Polanšek ABAljoša Beber IPIrena Mavrič Pleško

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Overview

Comparative study reveals column-based RNA extraction optimizes grapevine fanleaf virus detection in Xiphinema index nematodes, highlighting improved sampling protocols for vineyard management.

Key Points

To compare the efficacy of four sample preparation strategies for detecting grapevine fanleaf virus (GFLV) in its nematode vector, Xiphinema index, using RT-PCR.
Evaluated four sample preparation methods: column-based RNA extraction (RNeasy Plant Mini Kit), magnetic bead-based extraction (MagMAX RNA Isolation Kit), direct RT-PCR, and brief heat treatment.
Tested samples containing one, two, or five adult female X. index nematodes collected from three distinct vineyards.
Utilized transmission electron microscopy to confirm the presence of GFLV-like particles in the anterior region of individual nematodes.
Column-based RNA extraction demonstrated the highest detection probability and most consistent performance, successfully identifying GFLV in samples containing only one or two nematodes.
Detection success increased with higher nematode counts per sample, displaying a more pronounced sensitivity gain in methods that bypassed RNA extraction.

Cite This Study

Polanšek et al. (2026) studied this question.

synapsesocial.com/papers/6aa27b9a58559d80afc74da5 https://doi.org/10.1007/s10658-026-03311-5

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September 10, 2026European Journal of Plant PathologyOpen Access

Comparative analyses of molecular detection protocols for grapevine fanleaf virus (GFLV) in Xiphinema index

View Full Paper

Ask AI

Bookmark

Authors

JPJulija Polanšek ABAljoša Beber IPIrena Mavrič Pleško

Discussion

Member takes

Overview

Comparative study reveals column-based RNA extraction optimizes grapevine fanleaf virus detection in Xiphinema index nematodes, highlighting improved sampling protocols for vineyard management.

Key Points

To compare the efficacy of four sample preparation strategies for detecting grapevine fanleaf virus (GFLV) in its nematode vector, Xiphinema index, using RT-PCR.
Evaluated four sample preparation methods: column-based RNA extraction (RNeasy Plant Mini Kit), magnetic bead-based extraction (MagMAX RNA Isolation Kit), direct RT-PCR, and brief heat treatment.
Tested samples containing one, two, or five adult female X. index nematodes collected from three distinct vineyards.
Utilized transmission electron microscopy to confirm the presence of GFLV-like particles in the anterior region of individual nematodes.
Column-based RNA extraction demonstrated the highest detection probability and most consistent performance, successfully identifying GFLV in samples containing only one or two nematodes.
Detection success increased with higher nematode counts per sample, displaying a more pronounced sensitivity gain in methods that bypassed RNA extraction.

Cite This Study

Polanšek et al. (2026) studied this question.

synapsesocial.com/papers/6aa27b9a58559d80afc74da5 https://doi.org/10.1007/s10658-026-03311-5

View Full Paper

Ask AI

Bookmark