Samples of wheat leaves infected with leaf rust, caused by Puccina recondita f. sp. tritici, were collected in Uruguay in 1989, 1991, 1992, and 1993. The left rust collections were processed and evaluated for identification of virulence phenotypes on Tatcher wheat lines near-isogenic for leaf rust resistance genes. During the 4 yr, 37 virulence phenotypes were identified from a total of 316 single-uredinium isolates. The largest number of phenotypes were identified in 1989 when rust samples were collected from the near-isogenic
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Silvia Germán (1994) studied this question.