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In the study described in this report orthogonal Click reactions were utilized to immobilize two different proteins on surfaces side-by-side and in multilayer constructs. Alkyne- and azide-functionalized poly(ethylene glycol) hydrogel features were fabricated. Copper-catalyzed Huisgen 1,3 dipolar cycloaddition and oxime chemistry were employed to conjugate an azide-functionalized ubiquitin and oxoamide-modified myoglobin, respectively. Multicomponent patterning was verified by fluorescence imaging.
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Broyer et al. (2011) studied this question.
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