Profile of miR-10b Expression and Metastases-related Genes in BC and Fibro Adenoma in West Sumatera

Background and Objective: miR-10b is one of oncogenic miRNA first described in promoting metastases in Breast Cancer.The aim of the study was to investigate the expression of miR-10b and the expression of metastases-induced genes in BC (BC) and fibroadenoma (FA) in West Sumatra.Materials and Method: LNATM primer enhancer set was used to identify the miR-10b expression as a relative quantification.miR-16 was used as an endogenous control with a relative median expression of miR-10b at 2G ))Ct .The expression of metastases-related genes was performed by an absolute quantification method.Results: The statistical differences between miR-10b and the expression of genes were determined by t-student test to interpret the expression of BC and FA tissue (p<0.005).The expression of miR-10b in BC was lower than FA (endogenous control).Relative median of miR-10b expression in BC was 8.51 times lower than FA.Low expression of miR-10b in BC was associated with tissue grading.The expression of metastases-related gene; RhoC, TIMP2 and MMP2 were lower in BC than FA.miR-10b expression differed between BC and FA.Conclusion: RhoC, TIMP2 and MMP2 expression caused cancer cell whether to be metastasized or not.miR-10b is a potential marker to predict BC cell which start to be aggressive.