Caveolin-1 is dispensable for early lymphoid development, but plays a role in the maintenance of the mature splenic microenvironment

Figures

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  Fig. 1. Caveolin-1 deficiency is associated with splenomegaly with implications for Caveolin-1 as haploinsufficient for splenic B-cell maintenance. a Line graph of mean body weight (g) of Cav1+/+ (red), Cav1+/− (blue), and Cav1−/− (green) mice at indicated timepoint (n = 9–22 per group). b Line graph of mean SP weight (mg) of Cav1+/+ (red), Cav1+/− (blue), and Cav1−/− (green) mice at indicated timepoint (n = 9–15 per group). c (Top panel) Dot plots for CD19+CD22+ B cells in the SP for listed genotypes at 12, 24, and 36 weeks (n = 7–10 per group). (Bottom panel) Representative contour flow cytometry plots for CD19+CD22+ B cells at listed genotypes for listed timepoints. (Line graphs show mean ± SEM, dot plots show mean ± SD with each dot corresponding to a biological replicate, flow cytometry gate values show mean ± SEM, NS not significant, *p < 0.05 ANOVA and Tukey post hoc test)

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  Fig. 2. Aged Cav1-transgenic mice exhibit an altered splenic microenvironment. a Representative H&E stained sections of Cav1+/+, Cav1+/−, and Cav1−/− spleens at ×4 magnification. b Bar graph of the mean splenic secondary follicle area (μm2) for listed genotypes at 12 and 36 weeks (n = 5–10 follicles across n = 2–4 mice per group). c Percentage of observable secondary follicles for listed genotypes at 12 and 36 weeks (n = 2–4 per group). d Representative Ki67 and Movat’s Pentachrome stained histological sections from 36-week spleens for listed genotypes at 20× magnification (n = 3 per group). Increased fibrin staining is visualized in bright red. e Bar graph of mean plasma cells identified per field of view (×40) for listed genotypes from 36-week splenic sections (n = 3 per group). (Bar graphs show mean ± SD, with each dot corresponding to a biological replicate NS not significant, *p < 0.05 ANOVA and Tukey post hoc test)

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  Fig. 3. Follicle center B-cell lymphomas display altered CAV1 staining and expression patterns. a Representative tissue microarray cores stained for CAV1 expression. All images taken at ×20. b Bar graph of the distribution of composite scores assigned to healthy LN (n = 26), DBCL (n = 293), and FL (n = 18) samples for CAV1 staining. c Boxplot of the normalized log-transformed CAV1 expression value using the 212097_at signal probe for SP (n = 12), diffuse large B-cell lymphoma activated B cell-like (DLBCL-ABC) (n = 46), diffuse large B-cell lymphoma germinal center B cell-like (DLBCL-GCB) (n = 40), and FL (n = 27). Line within box represents median, whiskers extend to Tukey lines. (For (b): NS not significant, please see “Methods” for description of scoring key, *p < 0.05 Kruskal–Wallis and Dunn’s multiple comparisons test. For (c): NS not significant, *p < 0.001 comparison among groups of arrays method)