Energy-resolved mass spectra were recorded by controlled up-front collision-induced dissociation (CID) or conventional CID tandem mass spectrometry. Differentiation between constitutional isomers and stereoisomers of hydroxysteroids was studied using atmospheric pressure chemical ionization interfaced to a triple quadrupole mass spectrometer. Relative abundances of fragment ions from mainly water loss were used.
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Sjöberg et al. (1998) studied this question.