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Comparison of the frequency of rooting in the tobacco leaf segments inoculated with Agrobacterium tumefaciens harboring various combinations of rolB, rolC, ORF13 and ORF14 of TL-DNA of Ri plasmid (pRiHRI) revealed that the genes differ in their function to stimulate adventitious root induction. A single gene rolB induced roots, while rolC, ORF13 and ORF14 independently promoted the root induction by the rolB gene. The effects of these genes on the rolB-mediated rooting were in the order of ORF13>rolC≦ORF14.
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Aoki et al. (1999) studied this question.
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