Optimal Codon Choice Can Improve the Efficiency and Fidelity of N‐Methyl Amino Acid Incorporation into Peptides by In‐Vitro Translation

May the best codon win: N-methyl amino acids (N-Me AAs) are important modifications found in several nonribosomal peptide therapeutics. Previously, it was shown that three N-Me AAs are efficiently incorporated into peptides using a cell-free in-vitro translation system. Now, the incorporation of these N-Me AAs has been optimized by varying the template codon.