Identification and Genomic Analysis of vB_Pae_HLL23, a Novel Phage of Carbapenem-Resistant Pseudomonas aeruginosa

铜绿假单胞菌是临床常见的机会致病菌。近年来，噬菌体治疗已成为对抗铜绿假单胞菌感染的一种有希望的策略，特别是在抗生素过度使用导致耐药性增加的情况下。本研究从医院污水中分离出一种新型噬菌体vB_Pae_HLL23，对耐碳青霉烯假单胞菌(Pseudomonas aeruginosa, CRPA)具有显著的裂解作用。透射电镜显示，vB_Pae_HLL23属于肌病毒科。生物学特性表明，HLL23噬菌体潜伏期为10 min，裂解周期为80 min，每个细胞爆发大小为195 PFU，对温度和pH变化的耐受性增强。我们的研究结果表明，HLL23生物学特性稳定，对耐碳青霉烯类铜绿假单胞菌具有杀伤能力，具有制备成耐药性铜绿假单胞菌的抗菌剂的潜力。Pseudomonas aeruginosa is a common clinical opportunistic pathogen. In recent years, phage therapy has emerged as a promising strategy against P. aeruginosa infections, especially in the context of increased resistance due to antibiotic overuse. In this study, a novel phage, vB_Pae_HLL23, was isolated from hospital wastewater and showed significant lysis against carbapenem-resistant Pseudomonas aeruginosa (CRPA). Transmission electron microscopy showed that vB_Pae_HLL23 belongs to the Myoviridae family. Biological characterization showed that the HLL23 phage had a latency of 10 min, a lysis cycle of 80 min, a burst size of 195 PFU per cell, and enhanced tolerance to temperature and pH changes. Our results suggest that HLL23 has stable biological properties and killing ability against carbapenem-resistant Pseudomonas aeruginosa, and has the potential to be prepared as an antimicrobial agent for drug-resistant Pseudomonas aeruginosa.