Three new polyacetylenes (1–3), together with six known polyacetylenes (4–9), were isolated from a 95% aqueous ethanol extract of the Atractylodes japonica Koidz.ez Kitam. Their structures were elucidated by HR-ESI -MS and NMR techniques as well as comparison with those reported in literatures. The cytotoxic activity of 1–3 were also evaluated.
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