Key result
CNS delivery of rAAV2-vectored PrP(c)-specific scFv antibodies delayed the onset of prion pathogenesis and decreased PrP(sc) burden in susceptible mice.
Why the study?
Does CNS delivery of rAAV2-expressed PrP(c)-specific scFv antibodies delay disease onset in susceptible mice inoculated with infectious prions?
Population
Susceptible mice inoculated peripherally with infectious prions
Design
Preclinical
Authors
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Hypothesis-generating for AAV-scFv in prion models; does not support clinical use in humans.
Does CNS delivery of rAAV2-expressed PrP(c)-specific scFv antibodies delay disease onset in susceptible mice inoculated with infectious prions?
CNS delivery of vectored prion-specific single-chain antibodies delays prion disease onset in mice, suggesting a potential prophylactic approach for transmissible spongiform encephalopathies and other protein-misfolding diseases.
Wuertzer et al. (2008) studied Prion diseases. rAAV2 viral vector expressing PrP(c)-specific scFv antibodies was evaluated on Onset of prion pathogenesis (clinical signs, rotarod performance, incubation periods, and PrP(sc) burden). CNS delivery of rAAV2-vectored PrP(c)-specific scFv antibodies delayed the onset of prion pathogenesis and decreased PrP(sc) burden in susceptible mice.