Review reveals molecular and computational tools for circular RNA modifications, highlighting methods to decipher their regulatory functions.
Key Points
To review the functional significance of posttranscriptional modifications in circular RNAs and evaluate molecular and computational methods used to detect them.
Literature review summarizing known epitranscriptomic modifications detected on circular RNAs.
Evaluation of experimental sequencing workflows and bioinformatics approaches designed for mapping modifications on covalently closed RNA molecules.
Identified N6-methyladenosine (m6A), 5-methylcytosine (m5C), adenosine-to-inosine (A-to-I), and N1-methyladenosine (m1A) as the primary chemical marks currently documented on circular RNAs.
Synthesized molecular detection protocols and computational algorithms to establish methodological guidance for mapping circular RNA epitranscriptomics.