Enhanced biodegradation of high-density polyethylene microplastics: Study of bacterial efficiency and process parameters

As global microplastic (MP) pollution intensifies, sustainable and effective remediation methods are gaining interest due to the growing environmental and health implications. Microorganisms are demonstrating remarkable capabilities to degrade these polymers, offering a promising solution for reducing MP contamination. The aim of this study was to utilize bacteria for the degradation of high-density polyethylene (HDPE) MPs, specifically Comamonas testosteroni NCIMB 8955 , Bacillus firmus NCTC 10335 and Paenibacillus macquariensis NCTC 10419. During the incubation, bacterial growth, pH and carbohydrate concentration were monitored, and samples were taken to track MP weight loss and changes in surface morphology and functional groups. Gravimetric analysis revealed degradation efficiencies of 15.30%, 13.00%, and 12.29% for B. firmus NCTC 10335, P. macquariensis NCTC 10419, and C. testosteroni NCIMB 8955, respectively, over 30 days or less. Scanning electron microscopy (SEM) further confirmed degradation, revealing surface deterioration and biofilm formation. Energy dispersive X-ray spectroscopy (EDS) showed changes in the functional groups on the polymer surface, indicating an increase in the O/C molar ratio. Fourier-transform infrared spectroscopy (FTIR) revealed an increase in the carbonyl and vinyl indexes. The influence of temperature, MP size, and concentration on biodegradation was systematically studied using C. testosteroni NCIMB 8955, which demonstrated the highest degradation rate. The best result, i.e., a degradation efficiency of 21.81%, was achieved at 35ºC, with MP sizes between 20-100 µm, and a concentration of 200 mg/L. These findings highlight the importance of process parameters during biodegradation and the potential of C. testosteroni NCIMB 8955 in developing sustainable bioremediation approaches to mitigate microplastic pollution. • B. firmus, P. macquariensis and C. testosteroni were able to degrade HDPE MP particles • Degradation was confirmed through gravimetric, SEM, EDX and FTIR analyses • Biofilm formation, surface deterioration and changes in functional groups were found • Temperature, MP size and concentration influenced the degradation efficiency • The highest degradation efficiency (21.81%) was achieved with C. testosteroni