Approach to Quantitative Assessment of the Interaction of Nanocontainers Based on Natural Anionic Polysaccharides Cross-Linked with Divalent Metal Ions and Drug Doxorubicin

This study presents polymer-colloid nanocontainers based on water-soluble, drug, biocompatible, and biodegradable anionic polysaccharides such as sodium alginate and sodium hyaluronate cross-linked with calcium and magnesium ions. Doxorubicin characterized by fluorescent properties is used as a drug for immobilization by the prepared nanocontainers. An approach is proposed for the quantitative determination of the thermodynamic binding constants of doxorubicin with nanocontainers and linear polysaccharides, which is based on the ability of nanocontainers to quench the fluorescence of doxorubicin during interaction. The proposed approach allows us to quantitatively assess the ability of nanocontainers to retain doxorubicin immobilized.