Oxidative conversion of indoxyl or a substituted indoxyl into the corresponding indigoid dye is a chromogenic reaction suitable for application in cytochemical staining methods for the localization of cellular enzymes. The synthesis is described of a series of substituted indoxyl acetates for comparative testing as model chromogenic substrates for esterases, and for study of the potentialities of the general chromogenic principle.
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Holt et al. (1958) studied this question.
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