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Two biological specimens typical of 3-D samples studied by confocal microscopy were imaged at matched focal planes in conventional and confocal microscopes. A comparison of the effectiveness of optical sectioning was made between confocal microscopy and wide-field microscopy with computer deconvolution (using commercially available image restoration software). A simple algorithm for improving the depth discrimination of a confocal microscope is described and demonstrated.
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Cox et al. (1993) studied this question.
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