Reversed‐phase HPLC separation of ionic dyes (methylene blue, neutral red, methyl orange, and acid orange) with 1‐alkyl‐3‐methylimidazolium ionic liquids as the eluent additives was studied. The addition of ionic liquid to the eluent affects the resolution, peak shape and reproducibility of the retention of ionic solutes. The use of ionic liquids as the additives facilitates the separation of ionic dyes with a reversed‐phase HPLC. Baseline‐resolved separation of these dyes was achieved in 12 minutes when the mobile phase of acetonitrile‐water (28:72,v/v) contained 0.3‐2.0% (v/v) [emim][BF4]. The detection limits for the four dyes ranged from 4.45 to 10.0 μg/L for an injection volume of 20 μL. Apparently, ionic liquid is an effective mobile phase additive for the separation of ionic dyes in reversed‐phase HPLC separation.
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Chen et al. (2010) studied this question.
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