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Laser-light-scattering and electron-spin-resonance spectroscopies have been used to measure the translational and the rotational diffusion coefficients of human albumin. In the framework of the Debye-Stokes-Einstein theory, the two coefficients yielded two significantly different values for the hydrodynamics radius of the biomolecule. The discrepancy is discussed in connection with the involvement of the solvent internal degrees of freedom.
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Cannistraro et al. (1986) studied this question.
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