Urea and ammonia were estimated every day for 14 days in sterile cultures of Colpidium campylum. Qualitative tests for uric acid were also made. Ammonia production increased linearly with age of culture. Urea was produced only during the logarithmic phase of growth. The cause of the disappearance of urea is unknown. No uric acid was present at any time.
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Nardone et al. (1950) studied this question.
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