The present procedures were developed prior to a study of the changes in arginase activity in epidermal carcinogenesis because none of the existing methods was found to meet the minimal requirements for a good assay (1). Recent advances in the determination of arginase activity of purified liver extracts (2) and in the knowledge of metallic activation of arginase (3) have served as the basis for the modifications described herein.
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Eugene Roberts (1948) studied this question.
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