Sinanaerobacter Chloroacetimidivorans Sp. Nov., An Obligate Anaerobic Bacterium Isolated From Anaerobic Sludge

This is the authors' abstract. We don't add key points for this paper.

An obligate anaerobic bacterial strain (BAD-6T) capable of degrading acetochlor and butachlor was isolated from an anaerobic acetochlor-degrading reactor. Cells were Gram-positive, straight to gently curved rods with flagella. The major fermentation products in peptone-yeast (PY) broth were acetate and butyrate. The optimum temperature and pH for growth was 30 °C and 7.2−7.5, respectively. The major cellular fatty acids (>10%) were C14:0 FAME, C16:0 FAME and cyc-9,10-C19:0 DMA. Genome sequencing revealed a genome size of 4.80 Mb, a G+C content of 43.6 mol% and 4741 protein-coding genes. The most closely related described species on the basis of 16S rRNA gene sequences was Anaerovorax odorimutans NorPutT in the order Clostridiales of the class Clostridia with sequence similarity of 94.9 %. The nucleotide identity (ANI) value and digital DNA–DNA hybridization (dDDH) between the genomes of strain BAD-6T and Ana. odorimutans NorPutT are 70.9% and 15.9%, respectively. Based on the distinct differences in phylogenetic and phenotypic characteristics between strain BAD-6T and related species, Sinanaerobacter chloroacetimidivorans gen. nov., sp. nov. is proposed to accommodate the strain. Strain BAD-10T is the type strain (= CCTCC AB 2021092T = KCTC 72521T).