Incubation of washed white cells with phytohemagglutinin (PHA) had a stimulatory effect on the rate of C14O2 evolution from uniformly C14‐labeled glucose by the cells. The intensity of this effect depended not only on the concentration of PHA, but also on the duration of soaking of the cells in protein‐free medium for tissue culture before PHA was added and on the length of incubation of the soaked cells with PHA before glucose‐U‐C14 was added. This effect of PHA on the rate of glucose‐U‐C14 oxidation by the cells was essentially the same, whether white blood cells or peritoneal white cells were used.
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J Hrachovec (1966) studied this question.