Key result
In vitro murine virus diagnostics provide sensitive results while reducing laboratory animal use.
In vitro diagnostic methods like PCR offer sensitive and specific detection of murine viruses in biological materials, reducing the need for laboratory animals.
Introduction of microbiologically contaminated materials into mice can cause infections and distort research results. As counter measures, biological materials should be routinely screened prior to use, ideally by specific, sensitive, and reliable diagnostic methods. In this overview, we present a description of the currently used detection methods for murine viruses, including virus isolation in cell culture, the mouse antibody production (MAP) test, infant mouse bioassay and the PCR, that are relevant to routine health monitoring and summarize available data on their comparative sensitivities. Current diagnostic trends toward increased use of in vitro methods, apart from providing sensitive and specific results, contribute to refinement, replacement and reduction of the use of laboratory animals.
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Mahabir et al. (2014) conducted a review in Murine virus contamination in biological materials. Detection methods for murine viruses (e.g., PCR, cell culture, MAP test) was evaluated. In vitro diagnostic methods for detecting murine viruses provide sensitive and specific results while contributing to the refinement, replacement, and reduction of laboratory animal use.
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