The Urinary Glycoprotein Gp51 as a Clinical Marker for Interstitial Cystitis

No AccessJournal of UrologyClinical Urology: Original Articles1 Jun 1999THE URINARY GLYCOPROTEIN GP51 AS A CLINICAL MARKER FOR INTERSTITIAL CYSTITIS DOLORES SHUPP BYRNE, JOHN F. SEDOR, JOANNE ESTOJAK, KENNETH J. FITZPATRICK, ALLEN N. CHIURA, and S. GRANT MULHOLLAND DOLORES SHUPP BYRNEDOLORES SHUPP BYRNE , JOHN F. SEDORJOHN F. SEDOR , JOANNE ESTOJAKJOANNE ESTOJAK , KENNETH J. FITZPATRICKKENNETH J. FITZPATRICK , ALLEN N. CHIURAALLEN N. CHIURA , and S. GRANT MULHOLLANDS. GRANT MULHOLLAND View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)68800-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: GP51 is a urinary glycoprotein with a molecular weight of 51 kDa. This glycoprotein is produced and secreted by the transitional epithelium of the genitourinary tract, and has been isolated from human urine. Studies have demonstrated that GP51 levels are decreased in bladder biopsies of patients with interstitial cystitis. We evaluated urinary GP51 in a noninvasive manner as a clinical marker of interstitial cystitis. Materials and Methods: Urinary GP51 levels were measured using antigen inhibition enzyme-linked immunosorbent assay. In blinded fashion we analyzed for quantitative differences 24-hour urine samples of 36 patients with interstitial cystitis and 23 normal controls who were age matched within 5 years (mean age 47.3). We also evaluated GP51 in random urine specimens of 17 normal controls, 14 patients with interstitial cystitis and 11 subjects who had undergone cystectomy to determine whether urinary GP51 is mainly produced by the bladder, which is the site of interstitial cystitis. To ascertain the specificity of urinary GP51 to interstitial cystitis urine samples of 34 patients with other urological diseases were measured and compared with findings in the samples of 15 with interstitial cystitis. Results: Low GP51 levels appeared to be unique to the interstitial cystitis state compared to normal (p = 0.008). GP51 in patients with interstitial cystitis and in those who underwent cystectomy was lower (p <0.001) than in normal controls. These findings suggest that the major source of urinary GP51 is the bladder. Also, we observed lower GP51 levels in interstitial cystitis than in other urinary tract diseases (p <0.0001). Conclusions: Our study substantiates the possibility of using GP51 as a clinical marker for diagnosing interstitial cystitis by a noninvasive urinary assay. References 1 : Interstitial cystitis: a critique of current concepts with a new proposal for pathologic diagnosis and pathogenesis. Urology1997; 49, 5A: 14. Google Scholar 2 : Neuromediated inflammation of the urinary bladder: a new animal model for the study of cystitis. J. Urol.1996; 155: 432A. abstract 486. Google Scholar 3 : Autoantibodies in interstitial cystitis. J. Urol.1994; 151: 587. Link, Google Scholar 4 : Bladder surface glycosaminoglycans: an epithelial permeability barrier. J. Urol.1990; 143: 139. Link, Google Scholar 5 : Epithelial dysfunction in nonbacterial cystitis (interstitial cystitis). J. Urol.1991; 145: 732. Link, Google Scholar 6 : Functional and structural characteristics of the glycosaminoglycans of the bladder luminal surface. J. Urol.1987; 138: 433. Link, Google Scholar 7 : Ultrastructure of cell surface coat (glycocalyx) in rat urinary bladder epithelium. Cell Tiss. Res.1984; 158: 281. Google Scholar 8 : A methodology for the characterization of urinary glycosaminoglycans. J. Urol.1984; 131: 995. Google Scholar 9 : Urinary bladder glycoproteins of the rabbit: extraction, biochemical and immunological studies. Immunol. Invest.1985; 14: 41. Google Scholar 10 : Urinary tract glycoprotein tissue distribution and antigenic specificity. World J. Urol.1994; 12: 21. Google Scholar 11 : Antisera to a rabbit urinary tract antigen also react with human bladder and kidney tissue. J. Urol.1990; 143: 414. Link, Google Scholar 12 : Distribution in human tissues of an antibacterial urinary glycoprotein. J. Urol.1993; 149: 405A. abstract 771. Google Scholar 13 : Interaction of urinary Tamm-Horsfall protein and component of urothelial surface mucin (GP1) in urinary tract infections. J. Urol.1991; 145: 233A. abstract 82. Google Scholar 14 : Interaction of urinary tract glycoprotein (GP1) with enteric bacteria. J. Urol.1989; 141: 250A. abstract 321. Google Scholar 15 : Decrease in bladder mucosal glycoprotein after oophorectomy. J. Urol.1984; 132: 380. Abstract, Google Scholar 16 : Decreased expression of a glycoprotein component of bladder surface mucin (GPl) in interstitial cystitis. J. Urol.1994; 151: 343. Abstract, Google Scholar 17 : Urinary glycosaminoglycan excretion as a laboratory marker in the diagnosis of interstitial cystitis. J. Urol.1993; 149: 31. Link, Google Scholar 18 : Abnormal urothelial HLA-DR expression in interstitial cystitis. Clin. Exp. Immunol.1992; 87: 450. Google Scholar 19 : Mast cell involvement in interstitial cystitis. J. Urol.1987; 138: 746. Link, Google Scholar 20 : Interstitial cystitis: Increased sympathetic innervation and related neuropeptide synthesis. J. Urol.1992; 147: 587. Link, Google Scholar 21 : Summary of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases workshop on interstitial cystitis, National Institutes of Health, Bethesda, Maryland, August 28-29, 1987. J. Urol.1988; 140: 203. Abstract, Google Scholar From the Department of Urology, Thomas Jefferson University, Philadelphia, Pennsylvania© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byLOKESHWAR V, SELZER M, CERWINKA W, GOMEZ M, KESTER R, BEJANY D and GOUSSE A (2018) URINARY URONATE AND SULFATED GLYCOSAMINOGLYCAN LEVELS: MARKERS FOR INTERSTITIAL CYSTITIS SEVERITYJournal of Urology, VOL. 174, NO. 1, (344-349), Online publication date: 1-Jul-2005.SHUPP BYRNE D, SEDOR J, SOROUSH M, McCUE P and MULHOLLAND S (2018) INTERACTION OF BLADDER GLYCOPROTEIN GP51 WITH UROPATHOGENIC BACTERIAJournal of Urology, VOL. 165, NO. 4, (1342-1346), Online publication date: 1-Apr-2001. Volume 161Issue 6June 1999Page: 1786-1790 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information DOLORES SHUPP BYRNE More articles by this author JOHN F. SEDOR More articles by this author JOANNE ESTOJAK More articles by this author KENNETH J. FITZPATRICK More articles by this author ALLEN N. CHIURA More articles by this author S. GRANT MULHOLLAND More articles by this author Expand All Advertisement PDF downloadLoading ...