On the possibility of the visualization of the velocity distribution in biological samples using SAM

The effect of velocity changes in a biological sample on its image, obtained by using a reflection acoustic microscope, is discussed in the case of a distance between the sample and the lens smaller than the focal length. The effect of the shift of the V(z) curve, caused by a velocity change in the material of the sample, is assumed as the main source of changes in brightness in such images. The value of the shift of V(z) curve is determined theoretically and verified experimentally for layers of glycerol and aqueous glycerol solution. The possibility of interpreting acoustic images as the images of the velocity distribution in the sample is also discussed.