Sensitive screen-printed photoelectrochemical immunoassay based on flower-shaped ZnIn2S4 nanomaterials

Cucumber mosaic virus (CMV) has seriously affected the yield of crops and caused huge economic losses. By exploring the detection methods of CMV, CMV can be detected more accurately and rapidly. The introduction of screen-printed electrodes (SPE) has greatly promoted the development of photoelectrochemical (PEC) biosensors. Herein, a PEC biosensor based on flexible SPE was developed, to achieve sensitive detection of CMV. This work efficiently regulated the PEC reaction by conducting enzymatic biocatalytic precipitation (BCP) on ZnIn 2 S 4 , which is exemplified by a CMV-dependent sandwich immune reaction and subsequent alkaline phosphatase (ALP)-activated BCP response. The developed PEC sensor demonstrates excellent analytical performance in CMV detection, with detection limit of 0.001 ng mL -1 . The advantage of this work lies in the introduction of one-time SPE, which acts as a flexible electrode, greatly enhances the speed and sensitivity of detection, and is expected to stimulate further research on wearable flexible electronic sensing.