In Torenia stem segments cultured on a defined medium without phytohormones, in vitro flower formation was influenced by the physiological states of the explants. Endogenous contents of ABA, but not those of IAA, were closely correlated with the physiological states of the explants. Application of ABA (100 ng/ml) to the culture medium stimulated flower formation in the originally vegetative explants which otherwise had little flower-forming capacity. Thus, endogenous ABA seems to be one of the factors controlling the flower-forming capacity of Torenia stem segments. The highest rate of flower formation in the stem segments was obtained when endogenous contents of ABA (which resulted from both endogenously present and externally applied ABA) in the stem tissues was between 16 and 20 ng/g fresh weight.
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Tanimoto et al. (1985) studied this question.