Prolonged physiological studies using gas mixtures containing μ l/liter levels of a specific gas component (e.g. ethylene or propylene) require a flowthrough system to ensure that the plant or plant part under study is exposed to a relatively constant gas concn. This prevents tissue incorporations and/or emanations (e.g. biosynthesis of ethylene or CO 2 ) from altering the physiological effective gas concn (1).
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Mikal E. Saltveit (1978) studied this question.