Cytokine Gene Expression Alterations in Human Macrophages Infected by Leishmania major.

OBJECTIVE: ) to decipher cytokine gene expression alterations in host macrophages. MATERIALS AND METHODS: for 4 hours. RNA isolation was performed using TRIzol reagent. RNA sequencing was performed using the Illumina sequencing platforms. Gene expression analysis was performed using a Bioconductor DESeq2 package. RESULTS: , with an up-regulation of cytokines and mostly down-regulation of their receptors. CONCLUSION: and how the host cell responds to leishmaniasis.