A simple differential staining procedure for demonstrating infection within the leaf tissue by Pseudomonas tomato, Ps. lachrymans and Xanthomonas vesicatoria has been developed. It is based on (1) clearing of plant tissue with a mixture of glycerol, lactic acid, phenol and water; (2) treating the leaf tissue with boiling KOH; and (3) staining with aniline blue‐chloralhydrate. When observed under a light microscope, the bacteria appear dark blue, whereas the leaf tissue appears transparent and colourless.
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Bashan et al. (1981) studied this question.
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