Anodic Oxidation of Cladribine and In Situ Evaluation of DNA‐Cladribine Interaction

This is the authors' abstract. We don't add key points for this paper.

Cladribine (CLD), 2‐chlorodeoxyadenosine, was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry over a wide pH range. The oxidation of CLD is an irreversible, pH‐dependent with the transfer of two electrons and two protons mechanism, leading to the formation of a hydroxylated species which undergoes reversible redox reactions with the transfer of one electron and one proton. The in situ evaluation of DNA‐CLD interaction using a DNA‐electrochemical biosensor was investigated. The CLD interaction causes dsDNA structural modifications in a time‐dependent manner, but no DNA oxidative damage caused by CLD was observed.