The association between atherosclerosis and nonalcoholic fatty liver disease

Figures

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  Figure 1.. Flow chart of this study.

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  Figure 2.. Heat map and volcano map of differential genes. (A) Heat map. Red represents upregulation, blue represents downregulation, and white represents no significant change. Con = control group; treat = experimental group. (B) Volcano plot. The graph also includes 2 vertical dashed lines representing −1 and 1 and a horizontal dashed line representing the adjusted P-value of .05.

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  Figure 3.. The results of the GO and KEGG analyses of differential genes. (A) GO analysis bubble plot. (B) GO analysis cycle plot. (C) KEGG analysis bubble plot. (D) KEGG analysis cycle plot. The X-axis indicates the gene ratio of each term. The Y-axis indicates the pathway. The bubble size indicates the number of associated genes. The color of each bubble indicates the adjusted P-value: the redder the color, the higher the enrichment.

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  Figure 4.. Weighted gene correlation network analysis (WGCNA) of common genes. (A) Soft threshold analysis. (B) Module correlation. (C) Heat map of module-trait relationships. Con = control; treat = experimental group.

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  Figure 5.. Analysis of hub genes by WGCNA. (A) PPI network of the “ME blue” module. (B) Gene clustering of the module with a sore of 12.571.

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  Figure 6.. GO and KEGG analysis of the hub gene. (A) GO analysis of the hub gene. (B) KEGG analysis of hub genes. The darker the PPI network, the more critical the gene. The X-axis indicates the gene ratio of each term. The Y-axis indicates the pathway. The bubble size indicates the number of associated genes. The color of each bubble indicates the adjusted P-value: the redder the color, the higher the enrichment.

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  Figure 7.. Analysis of common genes between atherosclerosis and NAFLD from 3 public databases. (A) Venn diagram of common genes for atherosclerosis in 3 databases. (B) Venn diagram of genes common to NAFLD in 3 databases. (C) Venn diagram of genes common to atherosclerosis and NAFLD. (D) GO enrichment analysis of 208 common genes. (E) KEGG enrichment analysis of 208 common genes.

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  Figure 8.. PPI network and cluster analysis of 208 common genes. (A) PPI network of 208 common genes. The darker the PPI network, the more critical the gene. (B) Gene clustering of the module with a score of 10.5. (C) Gene clustering of the module with a score of 8.5.

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  Figure 9.. Enrichment analysis of the module with a score of 10.5. (A) GO analysis of the module with a score of 10.5. (B) KEGG analysis of the module with a score of 10.5.

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  Figure 10.. Enrichment analysis of the module with a score of 8.5. (A) GO analysis for module scoring 8.5. (B) KEGG analysis for module scoring 8.5.

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  Figure 11.. Venn diagram of the interactions between hub genes and common genes. Green is hub genes, blue is genes shared by AS and NAFLD in the 3 databases.

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  Figure 12.. LASSO regression model analysis. (A) LASSO regression modeling. The horizontal coordinate is the number of L1 paradigms, the vertical coordinate is the coefficient, and a line represents a gene. (B) Variable filtering. The dotted line represents the corresponding parameter lambda, and the parameter corresponding to the dotted line on the left (lambda. min) is usually chosen, corresponding to what the value above indicates how many genes have coefficients that can be left behind.