Reduced epidermal growth factor receptor expression in hypohidrotic ectodermal dysplasia and Tabby mice.

Patients with hypohidrotic ectodermal dysplasia (HED) and Tabby ( Ta ) mice lack sweat glands and there is compelling evidence that these phenotypes are caused by mutations in the same highly conserved but unidentified X-linked gene.Previous studies showed that exogenous epidermal growth factor (EGF) reversed the Ta phenotype but the EGF status in HED patients has not been studied at all.Studies reported herein investigated the hypothesis that the EGF signaling pathway is involved in HED/ Ta .Fibroblasts from HED patients had a two-to eightfold decrease in binding capacity for 125 I-labeled EGF, a decreased expression of the immunoreactive 170-kD EGF receptor (EGFR) protein, and a corresponding reduction in EGFR mRNA.Reduced expression of the EGFR also was observed in Ta fibroblasts and liver membranes.Other aspects of the EGF signaling pathway, including EGF concentration in urine and plasma, were normal in both HED patients and Ta mice.We propose that a decreased expression of the EGFR plays a causal role in the HED/ Ta phenotype.( J. Clin.Invest.1996.97:2426-2432.)Key words: epidermal growth factor • hypohidrotic ectodermal dysplasia • epidermal growth factor receptor • Tabby mouse • morphogenesis MethodsCase histories.RV is a 3-yr-old Hispanic male.This child presented in infancy with a classical picture of X-linked anhidrotic ectodermal dysplasia.The patient has a positive family history.His mother's sis